Isothermal Microcalorimetry Provides New Insight into Terrestrial Carbon Cycling

Isothermal microcalorimetry provides new insights into biofilm variability and dynamics.

The purpose of this study was to investigate a three-species in vitro biofilm with peri-implantitis-related bacteria for its variability and metabolic activity. Streptococcus sanguinis, Fusobacterium nucleatum, and Porphyromonas gingivalis were suspended in simulated body fluid containing 0.2% glucose to form biofilms on polished, protein-coated implant-grade titanium disks over 72 h using a fl...

New insights into chitosan-DNA interactions using isothermal titration microcalorimetry.

The interaction of chitosan with plasmid DNA was investigated as a function of pH, buffer composition, degree of deacetylation (DDA), and molecular weight (M(n)) of chitosan, using isothermal titration microcalorimetry (ITC). The Single Set of Identical Sites model was used to obtain the enthalpy of interaction, the binding constant, and the stoichiometry of binding. The chitosan-DNA interactio...

Standards in Isothermal Microcalorimetry

The main calorimetric principles used in isothermal microcalorimetry are briefly discussed. Different chemical calibration and test reactions are discussed, with a focus on reactions suitable for ambient conditions: reactions initiated by mixing of liquids (including titration microcalorimetry), dissolution of solid compounds and of slightly soluble gases, a photochemical process, and thermal p...

Mitophagy: New Insight into the Cardiovascular Aging  

Isothermal microcalorimetry in applied biology

Techniques of isothermal microcalorimetry have been much improved during the past two decades. In addition to their use in fundamental research, applications of practical importance have been established in some areas. However, no significant use of isothermal microcalorimetry has yet been seen in practical applications of biology, despite many methodological studies reported from that area. Th...